Document Type : Original Article

Authors

• Elaheh Piralaiy ¹
• Badrkhan Rashwan Ismael ²
• Gholamreza Hamidian

¹ Assistant Professor, Sports Physiology Department, Faculty of Physical Education and Sports Sciences, Tabriz University, Iran

² Ph.D student, Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran

Abstract

Introduction: A significant body of evidence supports the protective role of exercise training in the cardiovascular system. Therefore, this study aimed to investigate the effects of aerobic training on miR-195 expression in the heart tissue of Wistar rats.

methods: Twelve male Wistar rats aged 8 weeks and mean weight of 253±21 g were randomly divided into two groups: 1) control and 2) aerobic training. Aerobic exercise included running on a treadmill with a sound stimulus at a speed of 5-24 m/min for 10-60 minutes, five days a week for eight weeks. 48 After the last training session, all rats were anesthetized with ketamine xylazine solution, and heart and blood samples were taken from the mice. The expression level of miR-195 in heart tissue was measured using real-time PCR. Independent t-test was used using SPSS software at the significance level of (P<0.05).

Results: Statistical findings for miR-195 gene expression showed that aerobic training protocol increased the expression of miR-195 gene compared to the control group (p=0.001).

Conclusion: It seems that increased expression of the miR-195 gene through aerobic training reduces the expression of collagen isoforms. This increase in miR-195 also plays a role in physiologic hypertrophy induced by direct aerobic exercise in the regulation of the extracellular matrix.

Keywords

• Aerobic Exercise
• Wistar Rats
• miR-195